Fecal samples were kept at -20 degrees Celsius Fecal prep: The feces were thawed and 1g of wet weight was added to 1000 microlitre double distilled water and vortexed for 30secs with stuart SA8. Serial dilutions were made of the fecal homogenate from 10^-1 to -10 with double distilled water. These dilutions were titrated andContinue reading “Snake fecal analysis method notes”
Category Archives: Personal Snake Research Tips
Barnes Maze – Snake Specific
Always watch the videos and pretend to code a couple times before starting: The Barnes maze is used to observe spatial cognition and memory mainly in rice. From what I can tell the Holtzmann et al. (1999) study was the first to adapt this for snakes. They used a circular arena with 8 possible entrancesContinue reading “Barnes Maze – Snake Specific”
Simple Rstudio Code for Snake behaviour
acs=read.csv(file.choose())View(acs) enriched30<-acs$X30min.test[acs$environment==”enriched”] – new data set name <- Numerical Variable [treatment variable == “specific treatment you want to subset”] >shapiro.test(enriched30) to test for normality, column or subset name in the brackets >boxplot(enriched30, standard30) makes a box plot >wilcox.test(enriched30, standard30) non parametric test for two treatments >t.test(enriched30, standard30, mu=0, alternative=”two.sided”, paired = T, conf.level = 0.99)Continue reading “Simple Rstudio Code for Snake behaviour”
Arena Habituation – Snake specific
Always watch the videos and pretend to code a couple times before starting: I normally use a square or rectangular shaped arena with a large enough area that forces the snake to explore The snakes tend to move along the walls, this is called thigmotaxis therefore try to use lighting and cameras that can captureContinue reading “Arena Habituation – Snake specific”
Al3xandria 